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192.24 gene-expression biochips  (fluidigm)


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    Structured Review

    fluidigm 192.24 gene-expression biochips
    192.24 Gene Expression Biochips, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+expression+biochips/192+24+gene+expression+chips/10__1016_slash_j__aqrep__2025__102758-118-8-10
    Average 90 stars, based on 1 article reviews
    192.24 gene-expression biochips - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Immunological insights into the resistance of Nile tilapia strains to an infection with tilapia lake virus.
    Article Snippet: The emergence of viral diseases affecting fish and causing very high mortality can lead to the disruption of aquaculture production.. Recently, this occurred in Nile tilapia aquaculture where a disease caused by a systemic infection with a novel virus named tilapia lake virus (TiLV) caused havoc in cultured populations.. With mortality surpassing 90% in young tilapia, the disease caused by TiLV has become a serious challenge for global tilapia aquaculture.

    Article Title: Are bold-shy personalities of European perch (Perca fluviatilis) linked to stress tolerance and immunity? A scope of harnessing fish behavior in aquaculture.
    Article Snippet: After a 30 min incubation period at 37 ◦C, 43 μL TE buffer (Sigma-Aldrich/Merck, Germany) was added per sample. .. The multiplex quantitative PCR (qPCR) analyses were performed on two 48.48 Gene Expression biochips (Standard BioTools) using the BioMark HD system (Standard BioTools). .. First, each of the above 50 μL cDNA samples was diluted in SsoFast EvaGreen Supermix with Low ROX (BioRad, Germany) and 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools) to produce the sample mixes.

    Article Title: At Least Two Genes Encode Many Variants of Irak3 in Rainbow Trout, but Neither the Full-Length Factor Nor Its Variants Interfere Directly With the TLR-Mediated Stimulation of Inflammation
    Article Snippet: To this end, cDNAs were individually pre-amplified in 10 cycles using the PreAmp Master Mix (Fluidigm) and subsequently treated with exonuclease I (New England BioLabs) to degrade single-stranded oligonucleotide primers. .. Finally, multiplex RT-qPCR was conducted using the 48.48 Fluidigm Gene Expression biochips, which were first primed in the MX IFC Controller (Fluidigm). .. Then they were loaded with the pre-amplified cDNA samples and eventually analyzed using the Biomark HD system with the manufacturer's thermal protocol “GE Fast 48 × 48 PCR+Melt v2.pcl.” EvaGreen fluorescence dyes (Bio-Rad) served as DNA-binding reporter molecules to allow for the quantification of the amplified target fragments.

    Article Title: Transcriptomic Response of Differentiating Porcine Myotubes to Thermal Stress and Donor Piglet Age
    Article Snippet: Multiplex RT-qPCR was conducted using a Biomark HD system and EvaGreen fluorescence dyes (Bio-Rad, Hercules, CA, USA), as previously described [ , ]. .. In brief, 48.48 Fluidigm gene expression biochips were first primed in the MX integrated fluidic circuit (IFC) controller (Fluidigm, South San Francisco, CA, USA), before being loaded with the pre-amplified cDNA samples and eventually analyzed using a Biomark HD instrument (Fluidigm, CA, USA). .. The raw RT-qPCR results were retrieved with instrument-specific analysis software (v. 3.0.2; Fluidigm, CA, USA).

    Article Title: The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (Oncorhynchus mykiss) Stimulated with Aeromonas salmonicida
    Article Snippet: Multiplex RTqPCR was conducted using the Biomark HD system and EvaGreen fluorescence dyes (BioRad, Hercules, CA, USA), as previously described [15]. .. In brief, the 48.48 Fluidigm gene expression biochips were first primed in the MX integrated fluidic circuit (IFC) controller (Fluidigm, South San Francisco, CA, USA) before being loaded with the preamplified cDNA samples and eventually analysed using the Biomark HD instrument (Fluidigm, CA, USA). .. The raw RT-qPCR results were retrieved with instrument-specific analysis software (v. 3.0.2; Fluidigm, CA, USA).

    Expressing:

    Article Title: Immunological insights into the resistance of Nile tilapia strains to an infection with tilapia lake virus.
    Article Snippet: The emergence of viral diseases affecting fish and causing very high mortality can lead to the disruption of aquaculture production.. Recently, this occurred in Nile tilapia aquaculture where a disease caused by a systemic infection with a novel virus named tilapia lake virus (TiLV) caused havoc in cultured populations.. With mortality surpassing 90% in young tilapia, the disease caused by TiLV has become a serious challenge for global tilapia aquaculture.

    Ubiquitin Proteomics:

    Article Title: Immunological insights into the resistance of Nile tilapia strains to an infection with tilapia lake virus.
    Article Snippet: The emergence of viral diseases affecting fish and causing very high mortality can lead to the disruption of aquaculture production.. Recently, this occurred in Nile tilapia aquaculture where a disease caused by a systemic infection with a novel virus named tilapia lake virus (TiLV) caused havoc in cultured populations.. With mortality surpassing 90% in young tilapia, the disease caused by TiLV has become a serious challenge for global tilapia aquaculture.

    Multiplex Assay:

    Article Title: Are bold-shy personalities of European perch (Perca fluviatilis) linked to stress tolerance and immunity? A scope of harnessing fish behavior in aquaculture.
    Article Snippet: After a 30 min incubation period at 37 ◦C, 43 μL TE buffer (Sigma-Aldrich/Merck, Germany) was added per sample. .. The multiplex quantitative PCR (qPCR) analyses were performed on two 48.48 Gene Expression biochips (Standard BioTools) using the BioMark HD system (Standard BioTools). .. First, each of the above 50 μL cDNA samples was diluted in SsoFast EvaGreen Supermix with Low ROX (BioRad, Germany) and 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools) to produce the sample mixes.

    Article Title: At Least Two Genes Encode Many Variants of Irak3 in Rainbow Trout, but Neither the Full-Length Factor Nor Its Variants Interfere Directly With the TLR-Mediated Stimulation of Inflammation
    Article Snippet: To this end, cDNAs were individually pre-amplified in 10 cycles using the PreAmp Master Mix (Fluidigm) and subsequently treated with exonuclease I (New England BioLabs) to degrade single-stranded oligonucleotide primers. .. Finally, multiplex RT-qPCR was conducted using the 48.48 Fluidigm Gene Expression biochips, which were first primed in the MX IFC Controller (Fluidigm). .. Then they were loaded with the pre-amplified cDNA samples and eventually analyzed using the Biomark HD system with the manufacturer's thermal protocol “GE Fast 48 × 48 PCR+Melt v2.pcl.” EvaGreen fluorescence dyes (Bio-Rad) served as DNA-binding reporter molecules to allow for the quantification of the amplified target fragments.

    Real-time Polymerase Chain Reaction:

    Article Title: Are bold-shy personalities of European perch (Perca fluviatilis) linked to stress tolerance and immunity? A scope of harnessing fish behavior in aquaculture.
    Article Snippet: After a 30 min incubation period at 37 ◦C, 43 μL TE buffer (Sigma-Aldrich/Merck, Germany) was added per sample. .. The multiplex quantitative PCR (qPCR) analyses were performed on two 48.48 Gene Expression biochips (Standard BioTools) using the BioMark HD system (Standard BioTools). .. First, each of the above 50 μL cDNA samples was diluted in SsoFast EvaGreen Supermix with Low ROX (BioRad, Germany) and 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools) to produce the sample mixes.

    Quantitative RT-PCR:

    Article Title: At Least Two Genes Encode Many Variants of Irak3 in Rainbow Trout, but Neither the Full-Length Factor Nor Its Variants Interfere Directly With the TLR-Mediated Stimulation of Inflammation
    Article Snippet: To this end, cDNAs were individually pre-amplified in 10 cycles using the PreAmp Master Mix (Fluidigm) and subsequently treated with exonuclease I (New England BioLabs) to degrade single-stranded oligonucleotide primers. .. Finally, multiplex RT-qPCR was conducted using the 48.48 Fluidigm Gene Expression biochips, which were first primed in the MX IFC Controller (Fluidigm). .. Then they were loaded with the pre-amplified cDNA samples and eventually analyzed using the Biomark HD system with the manufacturer's thermal protocol “GE Fast 48 × 48 PCR+Melt v2.pcl.” EvaGreen fluorescence dyes (Bio-Rad) served as DNA-binding reporter molecules to allow for the quantification of the amplified target fragments.



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